1. Home
  2. Services
  3. Facilities
  4. Digital Pathology

Digital Pathology

Our Services
Metasystems qPCR system Next Generation Sequencing system Laser Microdissection system Histopathology Service Flow Cytometry Cell Irradiator Digital Pathology

Online core facilities booking system (for authorized personnel only)

A core service for digitalization of microscopic slides web-based virtual microscopy and automated image analysis.
 
This core service is provided by the Department of Pathology, The University of Hong Kong. It includes the scanning of whole microscopic slides in 20X or 40X resolution (of standard histological or immunohistochemical staining), hosting of digitalized slides for web-based virtual microscopy. This facility utilizes a Nanozoomer system S210 microscopic slide scanning system, coupled with software for web-based image storage and access. It allows user to utilize the following software applications for data analysis:

 

  • Tissue microarray analysis software – software for analysis of tissue microarray slides ( for department user only)
  • Positive pixel count – for quantification of area with positive staining and corresponding staining intensity
  • Nuclear algorithm – for quantification of nuclear staining (including counting % of nuclei with positive staining and corresponding staining intensity)
  • Membrane algorithm – for quantification of membranous staining (including counting % of cells with positive membranous staining and corresponding staining intensity)
  • Co-localisation algorithm – for quantification of two different colours (assessment of double staining, corresponding areas and intensity)
Service charges
26 mm × 76 mm slides  (Thickness 0.9 mm to 1.2 mm)
20x HKU User Non-HKU User
Ordinary slides

HK$20/slide

HK$40/slide
TMA mode (>20 cores)

HK$50/slide

HK$100/slide
     
40x HKU User Non-HKU User
Ordinary slides HK$40/slide HK$80/slide
TMA mode (>20 cores) HK$100/slide HK$200/slide
     

All charges include one-month slide hosting and web access.

For image analysis, freeware's can be use:
- aperio-imagescope (window only)
- Orbit (window, OSX, Linux)
- OMERO (window, OSX, Linux)
- QuPath (window, OSX, Linux)

Download Service Request Form

HKU Users

Non-HKU Users

Nanozoomer
Nanozoomer digital scan system

Nanozoomer digital scan system


email Mr. Kai Wong or Mr Andrew Lai  email 2255 2703 or 2255 2702 email hkuvirtualslide@pathology.hku.hk